IHC shows positive staining in paraffin-embedded human kidney. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
BSND Recombinant Rabbit mAb (S-3815-70)
BSND Recombinant Rabbit mAb (S-3815-70)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BSND |
| Synonyms | Barttin; BART |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q8WZ55 |
| Clone Number | S-3815-70 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| IF | 1:500 | Hu |
Background
BSND (Barttin CLCNK Type Beta Subunit) is a critical auxiliary subunit encoded by the BSND gene that is essential for the proper functioning of ClC-Ka and ClC-Kb chloride channels, which are predominantly expressed in the kidney's thick ascending limb of the loop of Henle and the stria vascularis of the inner ear. Unlike typical channel subunits that merely modulate gating, Barttin acts as an obligatory beta-subunit required for the trafficking of these alpha-subunits from the endoplasmic reticulum to the plasma membrane, thereby enabling chloride ion transport necessary for salt reabsorption and endolymph production. Loss-of-function mutations in the BSND gene disrupt this complex assembly, leading to impaired renal sodium-potassium-chloride cotransport and defective potassium recycling in the cochlea, which clinically manifests as autosomal recessive type IV Bartter syndrome characterized by severe antenatal polyhydramnios, profound salt-wasting nephropathy, metabolic alkalosis, and congenital sensorineural deafness, distinguishing it from other forms of Bartter syndrome that typically preserve hearing.
Picture
Picture
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human renal oncocytoma. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human chromophobe renal cell carcinoma. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human renal clear cell carcinoma. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human papillary renal cell carcinoma. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-BSND antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded Human kidney. Anti-BSND antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
