WB result of BSCL2/Seipin Recombinant Rabbit mAb
Primary antibody: BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 44 kDa
Observed MW: 48 kDa
BSCL2/Seipin Recombinant Rabbit mAb (S-1669-46)
BSCL2/Seipin Recombinant Rabbit mAb (S-1669-46)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BSCL2/Seipin |
| Synonyms | Bernardinelli-Seip congenital lipodystrophy type 2 protein |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum membrane |
| Accession | Q96G97 |
| Clone Number | S-1669-46 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms |
| Positive Sample | 293T, HeLa, mouse brain, mouse testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution,-20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IP | 1:50:00 |
Background
Seipin protein is a transmembrane protein that plays a crucial role in the biogenesis of lipid droplets (LDs). It directly affects cellular lipid homeostasis by regulating the mechanisms of fatty acid synthesis, phosphatidic acid (PA) metabolism, or sphingolipid production. Seipin mediates the synthesis of phosphatidylcholine (PC) and regulates lipolysis. Seipin has an evolutionarily conserved role in the biogenesis and degradation of lipid droplets across different species. The absence of Seipin can lead to severe diseases, including the genetic disorder Berardinelli-Seip congenital lipodystrophy type 2 (CGL2).
Picture
Picture
Western Blot
WB result of BSCL2/Seipin Recombinant Rabbit mAb
Primary antibody: BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 44 kDa
Observed MW: 48 kDa
IP
BSCL2/Seipin Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating BSCL2/Seipin in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: BSCL2/Seipin Recombinant Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 44 kDa
Observed MW: 48 kDa
