WB result of BSCL2/Seipin Recombinant Rabbit mAb
Primary antibody: BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Low expression control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 48 kDa
BSCL2/Seipin Recombinant Rabbit mAb (S-1669-135)
BSCL2/Seipin Recombinant Rabbit mAb (S-1669-135)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BSCL2/Seipin |
| Synonyms | Bernardinelli-Seip congenital lipodystrophy type 2 protein |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | Q96G97 |
| Clone Number | S-1669-135 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HEK-293, Raji, HeLa, MCF7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
BSCL2/Seipin is an endoplasmic-reticulum-anchored multi-pass transmembrane protein that drives proper lipid-droplet biogenesis by catalyzing the clustering and directed budding of nascent neutral-lipid lenses within the ER membrane, thereby acting as a molecular scaffold that recruits and concentrates diacylglycerol acyltransferase, phosphatidic-acid-metabolizing enzymes and lipin-1 to nascent droplet formation sites, while missense mutations that disrupt its N-glycosylation motif trigger ER stress, ubiquitin–proteasome degradation and conformational seipinopathy leading to congenital generalized lipodystrophy type 2 or dominant motor-neuron degenerations.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spinal cord. Anti-BSCL2/Seipin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
