WB result of Brd4 Recombinant Rabbit mAb
Primary antibody: Brd4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: K562 whole cell lysate 20 µg
Lane 6: 293T whole cell lysate 20 µg
Lane 7: Jurkat whole cell lysate 20 µg
Lane 8: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 160 kDa
Brd4 Recombinant Rabbit mAb (S-5145)
Brd4 Recombinant Rabbit mAb (S-5145)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Brd4 |
| Synonyms | Bromodomain-containing protein 4; HUNK1; BRD4 |
| Location | Nucleus |
| Accession | O60885 |
| Clone Number | S-5145 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | Raji, HepG2, A549, MCF7, K562, 293T, Jurkat, T-47D, NIH/3T3, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
| IHC-P | 1:250 | Hu |
Background
BRD4 (bromodomain-containing protein 4) is a nuclear protein belonging to the bromodomain and extra-terminal domain (BET) family, encoded by the BRD4 gene on human chromosome 19. This protein contains two highly conserved bromodomains (BD1 and BD2) and an extra-terminal domain (ET), enabling it to specifically recognize and bind acetylated histones and non-histone proteins. By recruiting positive transcription elongation factor b (P-TEFb) and other transcriptional regulatory complexes, BRD4 functions as a core coactivator in the process of transcriptional elongation. Under physiological conditions, BRD4 broadly regulates the expression of genes involved in cell cycle progression, proliferation, and differentiation, thereby maintaining genomic stability. However, in various malignancies (such as acute myeloid leukemia, triple-negative breast cancer, NUT midline carcinoma, etc.), BRD4 frequently forms BRD4-NUT fusion proteins through chromosomal translocation or is aberrantly recruited to super-enhancer regions, driving sustained high expression of oncogenes (e.g., MYC, BCL2) and promoting tumor progression. Recent studies have demonstrated that the functions of BRD4 extend beyond transcriptional regulation; it also participates in DNA damage repair, modulates autophagy, and regulates inflammatory responses. Meanwhile, small-molecule inhibitors targeting BRD4 (e.g., JQ1), which competitively block its binding to acetylated histones, have exhibited significant antitumor activity in numerous preclinical models. Furthermore, BRD4 plays an important role in cardiac development and maintenance of vascular endothelial function, and its expression dysregulation is closely associated with cardiovascular diseases such as cardiac hypertrophy and atherosclerosis. In summary, BRD4 serves as a critical hub linking epigenetic modifications and transcriptional regulation, holding great significance in both normal development and disease pathogenesis.
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Western Blot
WB result of Brd4 Recombinant Rabbit mAb
Primary antibody: Brd4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 160 kDa
WB result of Brd4 Recombinant Rabbit mAb
Primary antibody: Brd4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 160 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Brd4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Brd4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Brd4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
