WB result of BRCA2 Recombinant Rabbit mAb
Primary antibody: BRCA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MDA-MB-231 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 384 kDa
Observed MW: 280 kDa
BRCA2 Recombinant Rabbit mAb (S-1485-4)
BRCA2 Recombinant Rabbit mAb (S-1485-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BRCA2 |
| Synonyms | Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, FACD, FANCD1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P51587 |
| Clone Number | S-1485-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | HeLa, MDA-MB-231 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
The BRCA2 protein is a crucial component in maintaining genomic stability and is involved in various cellular functions such as homologous recombination, G2 checkpoint control, and protection of stalled replication forks. BRCA2 is also known to interact with numerous other proteins and has roles in DNA replication, including stabilizing stalled replication forks and protecting them from degradation. Mutations in the BRCA2 gene can lead to a deficiency in these repair mechanisms, which is why individuals with BRCA2 mutations are at an increased risk for developing certain types of cancer, particularly breast and ovarian cancer.
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Western Blot
