The BRAF activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | BRAF1, RAFB1 |
Accession | P15056 |
Amino Acid Sequence | E433-R726 |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 60.3 kDa |
Purity | > 35% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 20 mM Reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity


SDS-PAGE

M MW Standard
1 1 μg BRAF
1 3 μg BARF


