IHC shows positive staining in paraffin-embedded human chordoma. Anti-Brachyury antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Brachyury Recombinant Rabbit mAb (SDT-1926-107)
Brachyury Recombinant Rabbit mAb (SDT-1926-107)
Price:
Regular price
$100.00 USD
Regular price
Sale price
$100.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Brachyury |
| Synonyms | T-box transcription factor T; Brachyury protein; Protein T; T; TBXT |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | O15178 |
| Clone Number | SDT-1926-107 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
Brachyury protein is a transcription factor encoded by the TBXT gene, belonging to the T-box family of genes. It plays a crucial role in embryonic development, particularly in the formation and differentiation of the mesoderm and the notochord, which is the precursor of the spinal column. In adults, Brachyury is almost exclusively expressed in notochord-derived cells. Abnormal expression of Brachyury has been associated with certain cancers, such as chordoma, a malignant tumor that arises from residual notochordal cells. Additionally, it is involved in the epithelial-mesenchymal transition (EMT) in various carcinomas, promoting tumor invasion and metastasis.
Picture
Picture
Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human synovial sarcoma. Anti-Brachyury antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human fibrosarcoma. Anti-Brachyury antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Brachyury antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
