WB result of BMP2 Recombinant Rabbit mAb
Primary antibody: BMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Caco-2 whole cell lysate 20 µg
Lane 2: Saos-2 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 15, 45, 50 kDa
This blot was developed with high sensitivity substrate
BMP2 Recombinant Rabbit mAb (S-2312-45)
BMP2 Recombinant Rabbit mAb (S-2312-45)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BMP2 |
| Synonyms | Bone morphogenetic protein 2; BMP-2; Bone morphogenetic protein 2A (BMP-2A); BMP2A |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P12643 |
| Clone Number | S-2312-45 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Caco-2, Saos-2, LNCaP, C2C12, RAW264.7, mouse lung, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Bone Morphogenetic Protein 2 (BMP2) is a 32 kDa secreted signaling molecule belonging to the transforming growth factor-β superfamily that orchestrates osteogenesis, chondrogenesis, and embryonic patterning through binding to type I and type II serine/threonine kinase receptors, triggering Smad-dependent and Smad-independent pathways; recombinant human BMP2 is FDA-approved as an osteoinductive implant to promote spinal fusion and fracture repair, while dysregulated BMP2 expression is implicated in heterotopic ossification, pulmonary hypertension, and several cancers.
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Western Blot
WB result of BMP2 Recombinant Rabbit mAb
Primary antibody: BMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 15, 45, 50 kDa
This blot was developed with high sensitivity substrate
WB result of BMP2 Recombinant Rabbit mAb
Primary antibody: BMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 15, 50 kDa
This blot was developed with high sensitivity substrate
