Bmi1 Recombinant Rabbit mAb (S-5143)

Catalog Number: S0B60047 Application: WB, IHC-P Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	BMI-1
Synonyms	Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; PCGF4; RNF51; BMI1
Location	Cytoplasm, Nucleus
Accession	P35226
Clone Number	S-5143
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Mk
Positive Sample	HeLa, A549, K562, NCCIT, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Mk
IHC-P	1: 500	Hu

Background

BMI-1 (B-cell-specific Moloney murine leukemia virus integration site 1) is a critical polycomb group protein that functions as an essential component of the Polycomb Repressive Complex 1 (PRC1), playing a pivotal role in epigenetic gene silencing by catalyzing the monoubiquitination of histone H2A at lysine 119, thereby repressing key target genes such as the INK4a/ARF locus (which encodes tumor suppressors p16INK4a and p14ARF) to regulate cell cycle progression, cellular senescence, and stem cell self-renewal; beyond its normal physiological functions in development and hematopoiesis, BMI-1 is frequently overexpressed in various malignancies, where it drives tumorigenesis, promotes cancer stem cell maintenance, confers resistance to chemotherapy and radiation, and serves as a significant prognostic marker and potential therapeutic target across numerous cancer types including breast, lung, and colorectal cancers.

Picture

Western Blot

WB result of Bmi1 Recombinant Rabbit mAb
Primary antibody: Bmi1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 40 kDa

WB result of Bmi1 Recombinant Rabbit mAb
Primary antibody: Bmi1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 40 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Bmi1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-Bmi1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Bmi1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Bmi1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Bmi1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.