The Biotin-WNK3 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the Biotin-WNK3 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | PRKWNK3 |
Accession | Q9BYP7-1 |
Amino Acid Sequence | M1-D434, N-terminal 6x His and Avi tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 53.3 kDa |
Purity | >90% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | Avi Tag, His Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg Biotinylated WNK3
2 3 μg Biotinylated WNK3
