2μg (R: reducing condition, N:non-reducing condition).
Biotinylated TPBG/5T4 Fc&Avi Tag Protein, Human
Biotinylated TPBG/5T4 Fc&Avi Tag Protein, Human
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$430 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | 5T4 oncofetal antigen, Wnt-activated inhibitory factor 1 (WAIF1) |
| Accession | Q13641 |
| Amino Acid Sequence | Ser32-Ser355 with hIgG1 Fc&Avi Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 80-120kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE&HPLC |
| Conjugation | Biotin |
| Tag | Avi Tag, Human IgG1 Fc |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Jiang YQ, Ma XJ, Wang YM, Feng Y, Kong Y, Huang AL, Jin ZX, Ying TL, Wu YL. Human germline-like monoclonal antibody against 5T4 enables potent ADC and CAR-T therapies for solid tumors. Acta Pharmacol Sin. 2026 May;47(5):1326-1337. |
Background
TPBG/5T4 (trophoblast glycoprotein/5T4 oncofetal antigen) is an approximately 72 kDa type I transmembrane glycoprotein whose extracellular domain forms a horseshoe-shaped rigid core composed of eight leucine-rich repeats (LRRs), with an intracellular domain containing a PDZ-interaction motif; functionally, it drives cell migration and tumor metastasis by inhibiting canonical Wnt/β-catenin signaling while simultaneously activating noncanonical Wnt pathways, regulating the CXCL12/CXCR4 chemotactic axis, and promoting epithelial-mesenchymal transition (EMT). Clinically, 5T4 is highly expressed in approximately 80% of solid tumors while being restricted in normal adult tissues, making it an ideal target for tumor immunotherapy that has been exploited in the development of vaccines (Trovax), antibody-drug conjugates (ADCs), CAR-T/TCR-T cell therapies, and superantigen fusion proteins (Naptumomab), and is closely associated with cancer stem cell properties and poor prognosis in multiple malignancies.
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SDS-PAGE
SEC-HPLC
The purity of Biotinylated TPBG/5T4 Fc&Avi Tag Protein, Human is more than 95% determined by SEC-HPLC.
ELISA
Immobilized Anti-Human 5T4 Antibody (Naptumomab) at 1.0μg/mL (100μL/well) can bind Biotinylated TPBG/5T4 Fc&Avi Tag Protein, Human(Cat. No.UA016102) with EC50 of 1.74-2.45ng/mL.
Immobilized Biotinylated TPBG/5T4 Fc&Avi Tag Protein, Human(Cat. No.UA016102) at 1.0μg/mL (100μL/well) can bind Anti-Human 5T4 Antibody (Naptumomab) with EC50 of 4.19-6.78ng/mL.
