The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with
fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P43405 |
| Amino Acid Sequence | M1-N635; N-Biotinylated-Avi |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 74.3 KDa (74 KDa) |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM DTT, 10% glycerol. |
| Stability & Storage | -80℃ |
Guidelines
Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
Picture
Picture
Bioactivity
SDS-PAGE
Lane M: Marker
Lane 1: 2 μg,reduced Lane 2: 10μg, reduced
