1μg (R: reducing condition, N:non-reducing condition).
Biotinylated Nucleolin/NCL His&Avi Tag Protein, Human
Biotinylated Nucleolin/NCL His&Avi Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Protein C23 |
| Accession | P19338-1 |
| Amino Acid Sequence | Val2-Glu710 with His&Avi Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 90-100kDa (Reducing) |
| Purity | ＞85% by SDS-PAGE |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Yang L, Niu K, Wang J, Shen W, Jiang R, Liu L, Song W, Wang X, Zhang X, Zhang R, Wei D, Fan M, Jia L, Tao K. Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD. J Hepatol. 2024 Oct;81(4):651-666. |
Background
Nucleolin (NCL) is a crucial multifunctional nucleolar protein in eukaryotic cells, possessing nucleo-cytoplasmic shuttling capability. Its three distinctive structural domains enable it to induce chromatin decondensation by binding to histone H1 and to specifically recognize RNA sequences (with higher affinity for 5'-UUAGGG-3' repeats than their DNA counterparts). This protein not only participates in fundamental biological processes such as ribosome biogenesis, pre-rRNA transcription and processing, and transcriptional elongation regulation, but is also extensively involved in cell proliferation, apoptosis regulation, and stress response. Under pathological conditions, through its roles in tumorigenesis and viral infection, nucleolin has emerged as a significant potential target for anti-tumor and anti-viral therapies.
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SDS-PAGE
ELISA
Immobilized Biotinylated Nucleolin/NCL His&Avi Tag Protein, Human (Cat. No. UA011311) at 2.0μg/mL (100μL/well) can bind Nucleolin Rabbit Polyclonal Antibody (Cat. No. S0B0951) with EC50 of 1.01-1.19 μg/mL.
