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Biotinylated LILRB4/CD85K/ILT3 Fc&Avi Tag Protein, Human

Biotinylated LILRB4/CD85K/ILT3 Fc&Avi Tag Protein, Human

Catalog Number: UA011339 Brand: UA BIOSCIENCE
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$430 USD
$430 USD
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Product Details

Product Specification


Species Human
Synonyms LIR5,HM18,ILT-3
Accession Q8NHJ6
Amino Acid Sequence

Gln22-Glu259 with hIgG1 Fc&Avi Tag at the C-Terminus
Expression System HEK293
Molecular Weight 55-70kDa (Reducing)
Purity ＞95% by SDS-PAGE & > 95% RP-HPLC
Conjugation Biotin
Tag Avi Tag, Human IgG1 Fc
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Zhou Y, Li Y, He Q, Kong Z, Yu R, Yu X, Xie A. Uncovering the Associations of LILRB4 Genotypes With Parkinson's Disease: From Clinical Traits to Potential Pathologies. CNS Neurosci Ther. 2025 Jul;31(7):e70522.
2.Zhang N, Wang Q, Chen G, Liu L, Wang Z, Ma L, Liang Y, Wu J, Li X, Liu X, Zhou F. Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress. J Clin Invest. 2025 Aug 5;135(20):e183761. 
3.Wang H, Ji S, Zhang J, Li C, Meng X, Sun Y, Wang L, Luan H, Li F, Hui L, Li F, Wei S, Yu H, Li Z. LILRB4 specific overexpression in myeloid cells promotes tumor progression and immunosuppression in mouse models. Biochem Biophys Res Commun. 2025 Apr 1;755:151536.

Background

ILT3 (also known as LILRB4/CD85k/LIR-5) is an approximately 60 kDa transmembrane glycoprotein containing two immunoglobulin-like domains in its extracellular region and three ITIM inhibitory motifs in its intracellular region, with a secreted isoform detectable in serum. This protein is primarily expressed in myeloid cells such as monocytes and dendritic cells, as well as vascular endothelial cells. By recognizing MHC class I molecules and transmitting inhibitory signals, it negatively regulates immune cell activation and can induce the generation of CD8⁺ suppressor T cells, thereby promoting immune tolerance. Clinically, ILT3 is involved in suppressing transplant rejection, while the upregulation of its soluble form in the tumor microenvironment is associated with immune evasion, making it a potential target for immunotherapy.

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SDS-PAGE

1μg (R: reducing condition, N:non-reducing condition).

RP-HPLC

The purity of Biotinylated LILRB4/CD85K/ILT3 Fc&Avi Tag Protein, Human is more than 95% determined by RP-HPLC.

ELISA

Immobilized LILRB4/CD85k/ILT3 Recombinant Rabbit mAb (S-280-51) (Cat. No. S0B0349) at 1.0μg/mL (100μL/well) can bind Biotinylated LILRB4/CD85K/ILT3 Fc&Avi Tag Protein, Human (Cat. No.UA011339) with EC50 of 7.61-11.28 ng/mL.