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Biotinylated IL-2 His&Avi Tag Protein, Mouse

Biotinylated IL-2 His&Avi Tag Protein, Mouse

Catalog Number: UA016085 Brand: UA BIOSCIENCE
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$430 USD
$430 USD
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Product Details

Product Specification


Species Mouse
Synonyms TCGF, lymphokine, Interleukin 2
Accession P04351-1
Amino Acid Sequence

Ala21-Gln169 with His&Avi Tag at the C-Terminus
Expression System HEK293
Molecular Weight 20-30kDa (Reducing)
Purity ＞90% by SDS-PAGE
Conjugation Biotin
Tag His Tag, Avi Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. ZHAO Y, OGISHI M, PAL A, et al. Expanding the cytokine receptor alphabet reprograms T cells into diverse states[J]. Nature, 2025, 645(8082): 1039-1050.
2. HERNANDEZ R, PODER J, LAPORTE K M, et al. Engineering IL-2 for immunotherapy of autoimmunity and cancer[J]. Nature Reviews Immunology, 2022, 22(10): 614-628.
3. Raeber ME, Caspar DP, Zurbuchen Y, Guo N, Schmid J, Michler J, Martin AC, Steiner UC, Moor AE, Koning F, Boyman O. Interleukin-2 immunotherapy reveals human regulatory T cell subsets with distinct functional and tissue-homing characteristics. Immunity. 2024 Sep 10;57(9):2232-2250.e10.
4. CODARRI DEAK L, NICOLINI V, HASHIMOTO M, et al. PD-1-cis IL-2R agonism yields better effectors from stem-like CD8+ T cells[J]. Nature, 2022, 610(7930): 161-172.

Background

IL-2 (interleukin-2) is a four-helix bundle cytokine that signals through a high-affinity receptor complex comprising IL-2Rα, IL-2Rβ, and the common γ-chain (γc), activating the JAK1–JAK3 heterodimer and STAT5 pathway to regulate T cell development, differentiation, memory formation, and immune tolerance. Structurally, IL-2 forms a ternary complex with its receptor, and engineering of the binding interface enables biased signaling. Functionally, IL-2 exerts dual regulatory effects on effector T cells and regulatory T cells (Tregs). Clinically, low-dose IL-2 is used to expand Tregs in autoimmune diseases such as systemic lupus erythematosus, while engineered strategies—including biased IL-2 mutants, PD-1-cis IL-2R agonists, and orthogonal IL-2/IL-2Rβ systems—selectively activate tumor-specific CD8+ T cells, opening new avenues for cancer immunotherapy.

Picture

SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

ELISA

Immobilized Mouse IL-2 R alpha/CD25 Protein, His Tag at 5.0μg/mL (100μL/well) can bind Biotinylated IL-2 His&Avi Tag Protein, Mouse(Cat. No.UA016085) with EC50 of 3.30-8.20ng/mL.