1 3

Biotinylated IL-2 His Tag Protein, Human

Biotinylated IL-2 His Tag Protein, Human

Catalog Number: UA016086 Brand: UA BIOSCIENCE
Share:
Price:
$430 USD
$430 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms Interleukin-2, T-cell growth factor (TCGF), Aldesleukin
Accession P60568-1
Amino Acid Sequence

Ala21-Thr153 with His&Avi Tag at the C-Terminus
Expression System HEK293
Molecular Weight 15-20kDa (Reducing)
Purity ＞95% by SDS-PAGE,> 85% by HPLC
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Shouse AN, LaPorte KM, Malek TR. Interleukin-2 signaling in the regulation of T cell biology in autoimmunity and cancer. Immunity. 2024 Mar 12;57(3):414-428.
2.Arenas-Ramirez N, Woytschak J, Boyman O. Interleukin-2: Biology, Design and Application. Trends Immunol. 2015 Dec;36(12):763-777. 

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant Human IL-2 is a 15.5 kDa protein, containing 134 amino acid including one intrachain disulfide bond.

Picture

SDS-PAGE

1μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of Biotinylated IL-2 His Tag Protein, Human is more than 85% determined by SEC-HPLC.

ELISA

Immobilized IL-2R alpha/CD25 Fc Chimera Protein, Human(Cat. No. UA010260) at 5.0μg/mL (100μL/well) can bind Biotinylated IL-2 His Tag Protein, Human(Cat. No. UA016090) with EC50 of 3.67-5.89 ng/mL.