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Biotinylated Fc γ RIIIa/CD16a(F176) Avi&His Tag Protein, Human

Biotinylated Fc γ RIIIa/CD16a(F176) Avi&His Tag Protein, Human

Catalog Number: UA010425 Brand: UA BIOSCIENCE
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$594 USD
$594 USD
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Product Details

Product Specification


Species Human
Synonyms Fc γ RIIIa,CD16a,FcR-10
Accession P08637-1
Amino Acid Sequence

Gly17-Gln208, with C-terminal Avi & 8*His Tag
Expression System HEK293
Molecular Weight

45-53kDa
Purity >95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1、Nimmerjahn F. et al. (2006) Fcgamma receptors: old friends and new family members. Immunity J. 24(1): 19-28.

Background

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. A single nucleotide polymorphism creates high binding (V176) and low binding (F176) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

Guidelines


Picture

SDS-PAGE

1μg (R: reducing conditions, N: non-reducing conditions).

SPR

Immobilized Biotinylated Fc γ RIIIa/CD16a(F176) Avi&His Tag Protein, Human (Cat. No. UA010425) on SA Chip can bind Anti-CD20 Monoclonal Antibody (Rituximab) (Cat. No. S0B0570) with an affinity constant of 1.61 μM as determined in a SPR assay (Biacore T200).

Immobilized Biotinylated Fc γ RIIIa/CD16a(F176) Avi&His Tag Protein, Human (Cat. No. UA010425) on SA Chip can bind Anti-CD20 Monoclonal Antibody (Rituximab) (Cat. No. S0B0570) with an affinity constant of 1.61 μM as determined in a SPR assay (Biacore T200).