1μg (R: reducing condition, N: non-reducing condition).
Biotinylated CXADR His & Avi Tag Protein, Mouse
Biotinylated CXADR His & Avi Tag Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Coxsackievirus and adenovirus receptor homolog, CAR, Cxadr, CVB3 BP |
| Accession | P97792 |
| Amino Acid Sequence | Leu20-Gly237 with His Tag & Avi Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 30-40kDa (Reducing) |
| Purity | > 95% by SDS-PAGE & SEC-HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. |
Background
CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family within the immunoglobulin (Ig) superfamily. It plays a critical role in normal cardiac development in mice. Proposed to function as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex, essential for maintaining tight junction integrity and likely involved in the transepithelial migration of polymorphonuclear leukocytes (PMNs).
The mature mouse CXADR structure comprises a 218 amino acid (aa) extracellular domain (ECD), which includes a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment, and a 17 aa intracellular domain. Notably, D1 is believed to be responsible for homodimer formation within tight junctions. The ECD of mouse CXADR shares 97% sequence identity with the corresponding regions of human CXADR and 9% sequence identity with those of rat CXADR.
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SDS-PAGE
SEC-HPLC
The purity of Biotinylated CXADR His&Avi Tag Protein, Mouse is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized Recombinant Mouse JAML/AMICA Protein (His Tag), HPLC-verified at 2.0μg/mL (100μL/well) can bind Biotinylated CXADR His & Avi Tag Protein, Mouse (Cat. No. UA011134) with EC50 of 1.57-1.99 μg/mL.
