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Biotinylated CEACAM-5/CD66e (499-685) His&Avi Tag Protein, Human

Biotinylated CEACAM-5/CD66e (499-685) His&Avi Tag Protein, Human

Catalog Number: UA011362 Brand: UA BIOSCIENCE
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$470 USD
$470 USD
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Product Details

Product Specification


Species Human
Synonyms CEACAM-5, CEACAM, CD66e, CEA, Meconium antigen 100
Accession P06731-1
Amino Acid Sequence

Glu 499 - Ala 685 with His&Avi Tag at the C-Terminus
Expression System HEK293
Molecular Weight 43-55kDa (Reducing)
Purity ＞95% by SDS-PAGE&HPLC
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Li Q, Li Y, Li J, et al. FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis. Int J Biol Sci. 2018;14(7):726-735.
2. Baczyska D, et al. (2003) The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression. Cell Mol Biol Lett. 8(2): 471-86.
3. Blumenthal RD, et al. (2005) Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen). Cancer Res. 65(19): 8809-17.

Background

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

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SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of Biotinylated CEACAM-5/CD66e (499-685) His&Avi Tag Protein, Human is more than 95% determined by SEC-HPLC.

ELISA

Immobilized Biotinylated CEACAM-5/CD66e (499-685) His&Avi Tag Protein, Human (Cat. No. UA011362) at 2.0μg/mL (100μL/well) can bind Anti-Human CEA Monoclonal Antibody with EC50 of 0.50-0.70 ng/mL.

Immobilized Anti-Human CEA Monoclonal Antibody at 2.0μg/mL (100μL/well) can bind Biotinylated CEACAM-5/CD66e (499-685) His&Avi Tag Protein, Human(Cat. No. UA011362) with EC50 of 9.83-13.50 ng/mL.