1 3

Biotinylated CD8 alpha/CD8A His&Avi Tag Protein, Cynomolgus

Biotinylated CD8 alpha/CD8A His&Avi Tag Protein, Cynomolgus

Catalog Number: UA016044 Brand: UA BIOSCIENCE
Share:
Price:
$430 USD
$430 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Cynomolgus
Synonyms CD8, Leu2, MAL, p32
Accession XP_065382910.1
Amino Acid Sequence

Ser22-Asp182 with His&Avi Tag at the C-Terminus
Expression System HEK293
Molecular Weight

25-35kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Gao GF, Tormo J, Gerth UC, Wyer JR, McMichael AJ, Stuart DI, Bell JI, Jones EY, Jakobsen BK. Crystal structure of the complex between human CD8alpha(alpha) and HLA-A2. Nature. 1997 Jun 5;387(6633):630-4. 
2.Chen CY, Huang D, Wang RC, Shen L, Zeng G, Yao S, Shen Y, Halliday L, Fortman J, McAllister M, Estep J, Hunt R, Vasconcelos D, Du G, Porcelli SA, Larsen MH, Jacobs WR Jr, Haynes BF, Letvin NL, Chen ZW. A critical role for CD8 T cells in a nonhuman primate model of tuberculosis. PLoS Pathog. 2009 Apr;5(4): e1000392. 

Background

Cynomolgus macaque (Macaca fascicularis) CD8A shares high sequence homology with human CD8α (approximately 95%), and its CD8+ T cells are highly conserved with humans in terms of immunophenotype, signal transduction, and function. CD8α forms an Ig-like domain dimer that binds to the α3 domain of MHC class I molecules through CDR-like loops, thereby enhancing the recognition affinity of TCR for antigens. Cynomolgus CD8+ T cells serve as a critical model for evaluating HIV/SIV vaccines, immune checkpoint inhibitors (such as anti-PD-1/PD-L1), and bispecific antibodies (such as B7-H4×CD3). The cynomolgus macaque model is utilized to predict the toxicity (such as cytokine release syndrome) and efficacy of immunotherapeutic drugs, wherein CD8+ T cell depletion experiments represent the gold standard for validating vaccine protective mechanisms and the effectiveness of immunotherapy targets.

Picture

SDS-PAGE

1μg (R: reducing condition, N:non-reducing condition).

ELISA

Immobilized Biotinylated CD8 alpha/CD8A His&Avi Tag Protein, Cynomolgus(Cat. No. UA016044) at 0.5μg/mL (100μL/well) on Streptavidin precoated (0.5μg/well) plate, can bind CD8a Monoclonal Antibody (OKT8) with EC50 of 0.68-0.90 ng/mL.

Immobilized Biotinylated CD8 alpha/CD8A His&Avi Tag Protein, Cynomolgus (Cat. No. UA016044) at 0.5 μg/mL (100 μL/well) on Streptavidin precoated (0.5ug/well) plate, can bind CD8α Mouse mAb (SDT-1036-34) (Cat. No. S0B2307) with EC50 of 1.13-1.60 ng/mL.