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Biotinylated CD3E&CD3D Heterodimer, His, Avi Tag&Tag Free Protein, Human

Biotinylated CD3E&CD3D Heterodimer, His, Avi Tag&Tag Free Protein, Human

Catalog Number: UA016084 Brand: UA BIOSCIENCE
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$450 USD
$450 USD
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Product Details

Product Specification


Species Human
Synonyms CD3 epsilon&CD3 delta, CD3E & CD3D
Accession P07766-1(CD3E)、P04234-1(CD3D)
Amino Acid Sequence

Asp 23 - Asp 126 (CD3E) with His&Avi Tag at the C-Terminus;
Phe 22 - Ala 105 (CD3D) with no Tag
Expression System HEK293
Molecular Weight

15-25kDa (Reducing)

Purity ＞95% by SDS-PAGE & HPLC
Conjugation Biotin
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Tuning TCR complex recruitment to the T cell antigen coupler (TAC) enhances TAC-T cell function. Sci Rep. 2025 Feb 25;15(1):6769.
2. Gedeon P C, Schaller T H, Chitneni S K, et al. A rationally designed fully human EGFRvIII:CD3-targeted bispecific antibody redirects human T cells to treat patient-derived intracerebral malignant glioma. [J]. Clinical Cancer Research, 2018: clincanres.0126.2017.

Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of Biotinylated CD3E&CD3D Heterodimer, His,Avi Tag&Tag Free Protein, Human is more than 95% determined by SEC-HPLC.

ELISA

Immobilized Biotinylated CD3E&CD3D Heterodimer, His,Avi Tag&Tag Free Protein, Human (Cat. No.UA016084) at 1.0μg/mL (100μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1)) with EC50 of 1.37-2.03 ng/mL.

Immobilized Biotinylated CD3E&CD3D Heterodimer, His,Avi Tag&Tag Free Protein, Human (Cat. No. UA016084) at 1 μg/mL (100 μL/well) on Streptavidin at 1.0μg/mL (100μL/well) can bind NA/LE Mouse anti-human CD3 mAb (Cat. No.S0B0009) with EC50 of 2.43-5.41 ng/mL.

SPR

Biotinylated CD3E&CD3D Heterodimer, His, Avi Tag&Tag Free Protein, Human (Cat. No. UA016084) captured on Biotin CAP - Series S sensor Chip can bind NA/LE Mouse anti-human CD3 mAb with an affinity constant of 0.92nM as determined in a SPR assay.