1 1

Biotinylated CD3E&CD3D Fc, His, Avi tag&Fc, Flag, Avi tag Heterodimer Protein, Human

Biotinylated CD3E&CD3D Fc, His, Avi tag&Fc, Flag, Avi tag Heterodimer Protein, Human

Catalog Number: UA011360 Brand: UA BIOSCIENCE
Share:
Price:
$450 USD
$450 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms CD3 epsilon&CD3 delta, CD3E & CD3D, CD3 delta & CD3 epsilon
Accession P07766-1、P04234-1
Amino Acid Sequence

Asp 23 - Asp 126 (CD3E) with hIgG1 Fc Tag at the C-Terminus, followed by polyhistidine tag and Avi tag
Phe 22 - Ala 105 (CD3D) with hIgG1 Fc Tag at the C-Terminus, followed by Flag tag and Avi tag
Expression System HEK293
Molecular Weight

43-55kDa (Reducing)

Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag Avi Tag, His Tag, Flag Tag, Human IgG1 Fc
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Tuning TCR complex recruitment to the T cell antigen coupler (TAC) enhances TAC-T cell function. Sci Rep. 2025 Feb 25;15(1):6769.
2. Gedeon P C , Schaller T H , Chitneni S K , et al. A rationally designed fully human EGFRvIII:CD3-targeted bispecific antibody redirects human T cells to treat patient-derived intracerebral malignant glioma.[J]. Clinical Cancer Research, 2018:clincanres.0126.2017.

Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Picture

SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

ELISA

Immobilized Biotinylated CD3E &CD3D, Fc, His, Avi tag&Fc, Flag, Avi tag Protein, Human at 2.0μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate can bind NA/LE Mouse anti-human CD3 mAb (Cat. No. S0B0009) with EC50 of 2.77-5.76 ng/mL.