1μg (R: reducing condition, N: non-reducing condition).
Biotinylated BTN1A1/Butyrophilin His&Avi Tag Protein, Human
Biotinylated BTN1A1/Butyrophilin His&Avi Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Butyrophilin Subfamily 1 Member A1; BT; BTN |
| Accession | Q13410-1 |
| Amino Acid Sequence | Ala26-Arg242 with His & Avi Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 33-40kDa (Reducing) |
| Purity | > 90 % as determined by SDS-PAGE & > 85 % as determined by SEC-HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Sandstrom, A. et al. (2014) Immunity 40:490. |
Background
BTN1A1, also known as BTN, is a member of the immunoglobulin superfamily and the major protein linked to milk fat droplets. It may function as a cell surface receptor. Its B30.2 domain binds to xanthine oxidoreductase (XOR), stabilizing XOR's association with the milk fat globule membrane and playing a key role in milk fat globule secretion. This binding is conserved among BTN1A1 orthologs but isn't shared by BTN2A1 or BTN3A1. The B30.2 domain in butyrophilins can sense changes in intracellular phospho-antigen (pAg) levels, and its binding to pAg triggers gamma δ T cell activation. Moreover, BTN1A1 regulates milk lipid content and droplet size, and it can inhibit the proliferation and metabolism of CD4 and CD8 T-cells activated by anti-CD3 antibodies, as well as their secretion of IL2 and IFNG.
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SDS-PAGE
SEC-HPLC
The purity of Biotinylated BTN1A1/Butyrophilin His&Avi Tag Protein, Human is greater than 85% as determined by SEC-HPLC.
ELISA
Immobilized BTN1A1/Butyrophilin Monoclonal Antibody at 10μg/mL (100μL/well) can bind Biotinylated BTN1A1/Butyrophilin His&Avi Tag Protein, Human (Cat. No. UA011208) with EC50 of 1.91-7.75ng/mL.
