The Biotin-STUB1 activity was quantified using TR-FRET-based assay. The reaction was performed by incubating the Biotin-STUB1 protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 665nm/620 nm fluorescence signal ratio on BMG.
Biotin-STUB1 Avi&His Tag Protein,Human
Biotin-STUB1 Avi&His Tag Protein,Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CHIP |
| Accession | Q9UNE7-1 |
| Amino Acid Sequence | S23-R154; N-terminal His-TEV- Avi tag |
| Expression System | E.coli |
| Molecular Weight | 19.1 kDa |
| Purity | > 90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
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Bioactivity
SDS-PAGE
