The Biotin-BRG1 activity was detected using SPR technology. The Biotin-BRG1 protein was immobilized onto the SA chip coupled with neutravidin using labeled biotin. The varying concentration of Alpelisib was injected onto the Biotin- BRG1 protein surface and recorded the response signal. The kinetics fitting was as follows with a kD value of ~
5.96x10-9 M.
Biotin-SMARCA4 Protein, Human
Biotin-SMARCA4 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | BAF190A,BRG1,SNF2B,SNF2L4 |
| Accession | P51532-2 |
| Amino Acid Sequence | E658-E1361,with N-terminal 6x His-TEV-Avi tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 85.5 kDa |
| Purity | >90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
SEC-HPLC
