The Biotin-BRM activity was quantified using a SPR-based assay. The Biotin-BRM protein was immobilized onto the SA chip using labeled biotin. The varying concentration of FHD-286 was injected and flowed over onto the Biotin-BRM protein surface. And the response signal was recorded real time. The kinetics fitting was as follows with a kD value of ~ 4.29x10-9 M.
Biotin-SMARCA2 Protein, Human
Biotin-SMARCA2 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | BAF190B, SMARCA2, SNF2A, SNF2L2,BRM |
| Accession | P51531-2 |
| Amino Acid Sequence | E636-D1331,with N-terminal His-Avi tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 85.2 kDa |
| Purity | >90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
