Flow cytometric analysis of CD45R/B220 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either Biotin Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse/Human CD45R/B220 Antibody (Right panel) at 5μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse/Human CD45R/B220 Antibody (S-R519)
Biotin Rat Anti-Mouse/Human CD45R/B220 Antibody (S-R519)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD45R/B220 |
| Synonyms | Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc |
| Location | Cell membrane, Synapse |
| Accession | P06800、P08575 |
| Clone Number | S-R519 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu, Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu, Ms |
Background
CD45R/B220 is an isoform of the CD45 protein, specifically expressed on murine B-lineage cells, including pro-B, pre-B, and mature B cells, as well as a small subset of T cells and NK cells. It is a 220 kDa surface molecule and a member of the protein tyrosine phosphatase (PTP) family, which plays a crucial role in regulating lymphocyte development and antigen receptor signaling. B220 is commonly used as a pan B-cell marker in mice due to its restricted expression on B cells, making it a valuable tool in immunological research and flow cytometry.
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