Flow cytometric analysis of Mouse CD366 (TIM-3) expression on mouse CD3, CD28 antibody treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were either not stimulated (Left panel) or stimulated 3 days with anti-mouse CD3 and anti-mouse CD28 (Right panel) and then stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 and SDT Biotin Rat Anti-Mouse CD366 (TIM-3) antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse TIM-3 (CD366) Antibody (S-R478)
Biotin Rat Anti-Mouse TIM-3 (CD366) Antibody (S-R478)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | TIM-3 (CD366) |
| Synonyms | Hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3; Tim3; Timd3; Havcr2 |
| Location | Membrane |
| Accession | Q8VIM0 |
| Clone Number | S-R478 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | CD3, CD28 antibody treated C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
TIM-3 (CD366) is a trans-membrane immune checkpoint receptor in the Ig super-family whose extracellular domain binds phosphatidyl-serine, CEACAM-1 or galectin-9 ligands to trigger downstream signaling through its conserved cytoplasmic tyrosine motif, thereby attenuating T-cell, NK-cell and dendritic-cell activation while promoting regulatory T-cell function and myeloid tolerance, making its blockade a promising strategy for enhancing anti-tumor and anti-viral immunity across multiple cancers and chronic infections.
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