Flow cytometric analysis of Mouse Ly-6A/E expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with either Biotin Rat IgG2a, κ Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse Ly-6A/E Antibody (Right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse Ly-6A/E Antibody (S-3835)
Biotin Rat Anti-Mouse Ly-6A/E Antibody (S-3835)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly-6A/E |
| Synonyms | Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1 (SCA-1); T-cell-activating protein (TAP) |
| Location | Cell membrane |
| Accession | P05533、Q64253 |
| Clone Number | S-3835 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
Ly-6A/E, also known as Sca-1 (stem cell antigen-1), is an 18-kDa glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the murine Ly-6 multigene family, predominantly expressed on multipotent hematopoietic stem cells in adult mouse bone marrow and fetal liver, and is widely used as a marker for isolating these stem cells; beyond its stem cell association, Ly-6A/E is upregulated on activated T and B lymphocytes and appears to participate in T-cell receptor-mediated activation, myoblast proliferation and differentiation, bone formation, and interferon-induced immune responses, with expression levels varying between mouse strains carrying different Ly-6 haplotypes.
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