Flow cytometric analysis of mouse FR4 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD4 Antibody and either Biotin Rat IgG1, k Isotype Control (left panel) or Biotin Rat Anti-Mouse FR4 Antibody (right panel) at 2 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Rat Anti-Mouse FR4 Antibody (S-R705)
Biotin Rat Anti-Mouse FR4 Antibody (S-R705)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | FR4 |
| Synonyms | Sperm-egg fusion protein Juno; Folate receptor 4; Folate receptor delta (FR-delta); Folate-binding protein 3; IZUMO1 receptor protein JUNO; Folbp3; Folr4; Juno s; Izumo1r |
| Location | Cell membrane |
| Accession | Q9EQF4 |
| Clone Number | S-R705 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
Folate receptor 4 (FR4), also known as folate receptor delta or IZUMO1R, is a protein encoded by the FOLR4 gene in humans. It is a subtype of the receptor for the vitamin folic acid and is primarily expressed on CD4 T lymphocytes, with lower expression on CD8 T cells. FR4 is highly expressed in regulatory T cells (Tregs) and is also found in tumor-associated macrophages (TAMs) and T follicular helper cells (TFH cells). This protein plays a role in immune regulation, as its high expression can distinguish Tregs from other T cells. Additionally, FR4 is involved in the high-affinity binding and transport of folate and folate analogs across the cell membrane.
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