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Biotin Rat Anti-Mouse CD370 Antibody (7H11)

Biotin Rat Anti-Mouse CD370 Antibody (7H11)

Catalog Number: S0B8496 Application: FCM Reactivity: Ms Conjugation: Biotin Brand: Starter
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Product Details

Product Specification


Host Rat
Antigen CD370
Synonyms C-type lectin domain family 9 member A; Dendritic cell natural killer lectin group receptor 1; Dngr-1; Clec9a
Location Membrane
Accession Q8BRU4
Clone Number 7H11
Antibody Type Rat mAb
Isotype IgG2b,k
Application FCM
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Biotin
Physical Appearance Liquid
Storage Buffer

PBS pH7.4, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

CD370, also known as CLEC9A or DNGR-1, is a type II transmembrane glycoprotein expressed almost exclusively on human CD141⁺ (mouse CD8α⁺) dendritic cells and a minor subset of CD14⁺CD16⁻ monocytes; it functions as an endocytic pattern-recognition receptor that binds exposed filamentous actin on necrotic cell debris, triggering Syk–ITAM signaling that drives efficient cross-presentation of dead-cell antigens to cytotoxic T lymphocytes and thereby links necrosis sensing to initiation of adaptive immunity.

Picture

FC

Flow cytometric analysis of Mouse CD370 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with FITC Rat Anti-Mouse CD11c antibody and APC Rat Anti-Mouse CD8α either Biotin Rat IgG2b, κ Isotype Control (Left panel) or SDT Biotin Rat Anti- Mouse CD370 antibody (Right panel) at 5 μl/test followed by Sav-PE. Gated on CD8+ viable cells. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.