Flow cytometric analysis of Mouse CD275 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD45R/B220 Antibody and either Biotin Rat IgG2a, k Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse CD275 Antibody (right panel) at 0.5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD275 (B7-H2, B7-RP1, ICOS Ligand) Antibody (HK5.3)
Biotin Rat Anti-Mouse CD275 (B7-H2, B7-RP1, ICOS Ligand) Antibody (HK5.3)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD275 |
| Synonyms | ICOS ligand; B7 homolog 2 (B7-H2); B7-like protein Gl50; B7-related protein 1 (B7RP-1); LICOS; B7h; B7h2; B7rp1; Icosl; Icoslg |
| Location | Cell membrane |
| Accession | Q9JHJ8 |
| Clone Number | HK5.3 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 0.25μg per million cells in 100μl volume | Ms |
Background
ICOSL (CD275), also known as B7-H2, is a ~60 kDa transmembrane glycoprotein of the B7/immunoglobulin superfamily that delivers the key costimulatory signal to ICOS on activated T cells, thereby driving T-cell proliferation, Th1/Th2/Th17 cytokine secretion, germinal-center formation, plasma-cell differentiation and memory T-cell survival, while also undergoing reverse signaling into dendritic cells, B cells, endothelial cells or tumor cells to modulate adhesion, migration and cytokine output, and—uniquely in humans—additionally engaging CD28 and CTLA-4 to fine-tune both naïve and regulatory T-cell responses, positioning it as a central checkpoint in adaptive immunity, allergy, autoimmunity and cancer.
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