Flow cytometric analysis of Mouse CD25 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD4 Antibody and SDT Biotin Rat Anti- Mouse CD25 Antibody at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD25 Antibody (S-R441)
Biotin Rat Anti-Mouse CD25 Antibody (S-R441)
Price:
Regular price
$143.00 SGD
Regular price
Sale price
$143.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2ra; Il2r |
| Location | Membrane |
| Accession | P01590 |
| Clone Number | S-R441 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.02mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
CD25, also known as the interleukin-2 receptor alpha chain (IL-2Rα), is a protein that serves as a receptor for interleukin-2 (IL-2), a cytokine that plays a crucial role in immune system regulation. CD25 is expressed on the surface of various immune cells, including T cells, B cells, natural killer (NK) cells, dendritic cells, and monocytes. The main function of CD25 is to bind to IL-2, which then triggers a signaling cascade that leads to the activation, proliferation, and differentiation of immune cells. CD25 expression is tightly regulated and is often used as a marker for regulatory T cells (Tregs), a subset of T cells that play a key role in suppressing immune responses and maintaining immune homeostasis. Tregs are characterized by high expression levels of CD25 and the transcription factor FoxP3. Abnormalities in CD25 expression or function have been linked to various immunological disorders. For example, deficiencies in CD25 expression or function can lead to immunodeficiency, while excessive CD25 expression has been associated with autoimmune diseases and cancer.
Picture
Picture
FC
