Flow cytometric analysis of CD205 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Pacific Blue™ Rat Anti-Mouse Ly-6G/Ly-6C Antibody and either Biotin Rat IgG2a, κ Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse CD205 Antibody (right panel) at 2 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Rat Anti-Mouse CD205 Antibody (NLDC-145)
Biotin Rat Anti-Mouse CD205 Antibody (NLDC-145)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD205 |
| Synonyms | Lymphocyte antigen 75; Ly-75; DEC-205; Cd205; Ly75 |
| Accession | Q60767 |
| Clone Number | NLDC-145 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD205, also known as DEC-205, is a type C lectin receptor and a type I membrane glycoprotein belonging to the mannose receptor family. It is highly expressed on dendritic cells (DCs), thymic epithelial cells, and certain epithelial cells. CD205 plays a crucial role in antigen uptake, processing, and presentation, facilitating the induction of antigen-specific T cell immunity. This receptor is involved in endocytosis, capturing antigens from the extracellular space and transporting them to specialized compartments for processing. Its high efficiency in antigen presentation makes it a valuable target in immunotherapy, particularly for developing vaccines against viruses and tumors. Additionally, CD205 has been found to recognize apoptotic and necrotic cells in an acidic environment through its interaction with keratin.
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