Flow cytometric analysis of Mouse CD163 expression on fresh C57BL/6 mouse splenocytes. Fresh C57BL/6 mouse bone marrow were stained with Pacific Blue™ Rat Anti-Mouse F4/80 Antibody and either Biotin Rat IgG2a, κ Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse CD163 Antibody (Right panel) at 5 μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD163 Antibody (S-R608)
Biotin Rat Anti-Mouse CD163 Antibody (S-R608)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD163 |
| Synonyms | Cd163 |
| Location | Cell membrane |
| Accession | B7ZMW6 |
| Clone Number | S-R608 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | Fresh C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD163 is a 130 kDa type I transmembrane glycoprotein and a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is exclusively expressed on monocytes and macrophages and is involved in the clearance and endocytosis of hemoglobin-haptoglobin complexes, which helps protect tissues from oxidative damage caused by free hemoglobin. The protein consists of nine SRCR domains, a single transmembrane segment, and a short cytoplasmic tail. CD163 is upregulated in macrophages during the switch to alternative activated phenotypes in inflammation and is considered a marker for anti-inflammatory macrophages. Elevated levels of soluble CD163, which result from the shedding of the membrane-bound form, have been observed in various acute and chronic inflammatory disorders, making it a potential biomarker for inflammation. Additionally, CD163 is being explored as a therapeutic target for delivering drugs to macrophages in inflammatory conditions.
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