Flow cytometric analysis of CD144 expression on bEnd.3 cells. Cells from the bEnd.3 (Mouse brain endothelioma, right) or NIH/3T3 (Mouse embryonic fibroblast, left) was stained with either Biotin Rat IgG1 κ Isotype Control (black line histogram) or SDT Biotin Rat Anti-Mouse CD144 Antibody (red line histogram) at 2 μl/test followed by Sav-APC. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD144 Antibody (S-R719)
Biotin Rat Anti-Mouse CD144 Antibody (S-R719)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD144 |
| Synonyms | Cadherin-5; Vascular endothelial cadherin (VE-cadherin); Cdh5 |
| Location | Cytoplasm, Cell membrane |
| Accession | P55284 |
| Clone Number | S-R719 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | bEnd.3 |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD144, also known as VE-cadherin or cadherin-5, is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail, expressed almost exclusively at the adherens junctions of endothelial cells in blood and lymphatic vessels, where it mediates homophilic adhesion, controls endothelial permeability, maintains vascular integrity, promotes endothelial quiescence and tubulogenesis, and is indispensable for embryonic vascular development since its genetic deletion or truncation causes mid-gestational lethality due to vessel disassembly and hemorrhage.
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