Flow cytometric analysis of Mouse CD14 expression on RAW 264.7 Cells from the RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) or Neuro-2a (Mouse neuroblastoma neuroblast, Left) was stained with Biotin Rat IgG2a, κ Isotype Control (Black line histogram) and SDT Biotin Rat Anti-Mouse CD14 Antibody (Red line histogram) at 5 μl/test followed by Sav-iFluor 488, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD14 Antibody (S-R609)
Biotin Rat Anti-Mouse CD14 Antibody (S-R609)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD14 |
| Synonyms | Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Cd14 |
| Location | Cell membrane |
| Accession | P10810 |
| Clone Number | S-R609 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | RAW 264.7 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD14 is a 375-amino-acon, pattern-recognition glycoprotein that circulates in plasma as a soluble 55 kDa fragment (sCD14) shed by GPI-anchor cleavage or exists as a 55 kDa, glycosylphosphatidylinositol-anchored membrane receptor (mCD14) on myeloid cells; in both forms it acts as the critical co-receptor for Toll-like receptor 4/MD-2, enabling innate immune detection of lipopolysaccharide and other bacterial lipoglycans, thereby triggering NF-κB–driven cytokine storms, and its serum concentration rises acutely in sepsis, making it both a biomarker and a therapeutic target for endotoxin-driven inflammation.
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