Flow cytometric analysis of CD107b expression on C57BL/6 mouse BMDMs. C57BL/6 mouse BMDMs were stained with PE Rat Anti-Mouse F4/80 Antibody and either Biotin Rat IgG1, κ Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse CD107b Antibody (right panel) at 2 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD107b Antibody (M3/84)
Biotin Rat Anti-Mouse CD107b Antibody (M3/84)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD107b |
| Synonyms | Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B (LGP-B); Lamp2 |
| Location | Lysosome, Endosome, Cell membrane |
| Accession | P17047 |
| Clone Number | M3/84 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse BMDM |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD107b, also known as lysosome-associated membrane protein 2 (LAMP-2), is a highly glycosylated type I transmembrane protein with a molecular weight of approximately 105–120 kDa. It is primarily located in the lysosomal/endosomal membranes of nearly all cells and can be transiently expressed on the surface of activated platelets, activated lymphocytes, and some tumor cell lines. LAMP-2 plays crucial roles in various cellular processes, including cell adhesion, cellular homeostasis, autophagocytosis, antigen presentation, and protection of the lysosome. Additionally, it may be involved in tumor cell metastasis by mediating adhesion to vascular endothelium.
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