Flow cytometric analysis of mouse CD117 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 421™ Rat Anti-Mouse Lineage Cocktail Antibody and either Biotin Rat IgG2b, κ Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse c-Kit (CD117) Antibody (right panel) at 2μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse c-Kit (CD117) Antibody (S-2755)
Biotin Rat Anti-Mouse c-Kit (CD117) Antibody (S-2755)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | c-Kit (CD117) |
| Synonyms | Mast/stem cell growth factor receptor Kit; SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; Sl; Kit |
| Location | Cytoplasm, Cell membrane |
| Accession | P05532 |
| Clone Number | S-2755 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
c-Kit (CD117) is a receptor tyrosine kinase encoded by the c-kit gene. It plays a crucial role in the development and function of various cell types including hematopoietic cells, melanocytes, and gastrointestinal interstitial cells. The ligand for c-Kit is stem cell factor (SCF), and the binding of SCF to c-Kit activates intracellular signaling pathways that promote cell proliferation, survival, differentiation, and migration. Mutations or overexpression of c-Kit are associated with several diseases such as gastrointestinal stromal tumors (GISTs) and certain types of leukemia, making it an important target for therapeutic interventions.
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