Flow cytometric analysis of TOX expression in C57BL/6 mouse thymocytes. C57BL/6 mouse thymocytes were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Pacific Blue™ Rat Anti- Mouse CD8 Antibody and either Biotin Rat IgG1, k Isotype Control (left panel) or SDT Biotin Rat Anti- Human/Mouse TOX Antibody (right panel) at 5 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Human/Mouse TOX Antibody (S-4516)
Biotin Rat Anti-Human/Mouse TOX Antibody (S-4516)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | TOX |
| Synonyms | Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; KIAA0808 |
| Location | Nucleus |
| Accession | O94900 |
| Clone Number | S-4516 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Hu, Ms |
| Positive Sample | C57BL/6 mouse thymocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Ms |
Background
TOX protein is a transcription factor that plays a crucial role in the immune system. It is involved in the regulation of T cell differentiation and function, particularly in the development and maintenance of regulatory T cells (Tregs) which are essential for maintaining immune tolerance and preventing autoimmunity. Additionally, TOX has been implicated in the response of T cells to chronic antigen stimulation, such as in the context of chronic infections or cancer, where it may contribute to T cell exhaustion. Its expression and activity can influence the balance between effector and regulatory T cell populations, thereby having a significant impact on the overall immune response.
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