Flow cytometric analysis of Mouse NK1.1/CD161 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Phycoerythrin Rat Anti-Mouse CD49b Antibody and either Biotin Isotype Control (Left panel) or SDT Biotin Rabbit Anti-Mouse NK1.1/CD161 Antibody (Right panel) at 5μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rabbit Anti-Mouse NK1.1/CD161 Antibody (S-428-32)
Biotin Rabbit Anti-Mouse NK1.1/CD161 Antibody (S-428-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NK1.1/CD161 |
| Synonyms | Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c (Ly-55c); NKR-P1.9; NKR-P1C; Natural killer cell surface protein P1-40 (NKR-P1 40); CD161c; Ly55c; Nkrp1c; Klrb1c |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P27814 |
| Clone Number | S-428-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
NK1.1/CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor expressed as a disulfide-linked homodimer on the cell membrane. It is predominantly found on the majority of natural killer (NK) cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, particularly those with a "memory" antigenic phenotype. The expression of CD161 is specifically upregulated by IL-12 and is associated with the cytotoxic function of CD16+ NK cells. Functionally, CD161 plays a role in modulating the activation and proliferation of NK cells, inducing interferon-γ production, and releasing cytotoxic granules. When CD161 binds to its ligand LLT1, it inhibits NK cell cytotoxicity and cytokine secretion.
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