Flow cytometric analysis of IL-2 expression in C57BL/6 mouse splenocytes stimulated with PMA and Ionomycin in the presence of Brefeldin A for 6hr (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT Biotin Rabbit Anti-Mouse IL-2 Antibody at 0.2 μg/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rabbit Anti-Mouse IL-2 Antibody (S-2117-6)
Biotin Rabbit Anti-Mouse IL-2 Antibody (S-2117-6)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P04351 |
| Clone Number | S-2117-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B8273 |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | PMA stimulated C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 0.2μg per million cells in 100μl volume | Ms |
Background
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
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