Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte, left) / A431 (Human epidermoid carcinoma epithelial cell, right) stained with Biotin Rabbit IgG Isotype Control (black line histogram) and SDT Biotin Rabbit Anti-Human CD44v6 Antibody (red line histogram) at 5 μl/test. Cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Negative control: Raji
Biotin Rabbit Anti-Human CD44v6 Antibody (S-944-13)
Biotin Rabbit Anti-Human CD44v6 Antibody (S-944-13)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD44v6 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16070 |
| Clone Number | S-944-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B8273 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | A431 |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD44 variable alternative exon 6 (CD44 V6) is a variant isoform of the CD44 gene resulting from alternative splicing of RNA. CD44 is a cell adhesion molecule that is present in various cell types and plays crucial roles in biological processes such as cell migration, proliferation, and differentiation. The CD44 V6 isoform specifically, has been implicated in tumor development and metastasis. Research has shown that CD44v6 is expressed by all colorectal cancer stem cells (CR-CSCs). The expression of CD44v6 is essential for the migration and formation of metastatic tumors, achieved through the activation of the WNT-β-catenin signaling pathway. Propelling evidence recommends that CD44v6 compared to CD44 has a more specific expression in CSCs and may have a higher diagnostic value.
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