Flow cytometric analysis of Human CD127 expression on Human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either Biotin Rabbit IgG Isotype Control (Left panel) or SDT Biotin Mouse Anti- Human CD127antibody (Right panel) at 1.25 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rabbit Anti-Human CD127 Antibody (S-1010-20)
Biotin Rabbit Anti-Human CD127 Antibody (S-1010-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD127 |
| Synonyms | Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; IL7R |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16871 |
| Clone Number | S-1010-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD127, also known as the interleukin-7 receptor alpha (IL7R-α), is a type I cytokine receptor encoded by the IL7R gene in humans. It is a subunit of the functional interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. CD127 is expressed on the surface of hematopoietic cells, particularly lymphoid lineage cells, and plays a crucial role in the development, survival, and function of T cells. It is involved in signaling pathways that promote cell survival and proliferation through the activation of JAK1, JAK3, STAT5, and the PI3K-AKT pathway. Additionally, CD127 exists in both membrane-bound and soluble forms, with the soluble form (sCD127) potentially having antagonistic or agonistic effects on IL-7 activity depending on the context.
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