Flow cytometric analysis of Mouse XCR1 expression on collagenase-digested C57BL/6 mouse splenocytes. Collagenase-digested C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD8 antibody and either Biotin Mouse IgG Isotype Control (Left panel) or SDT Biotin Mouse Anti- Mouse XCR1 antibody (Right panel) at 5 μl/test followed by Sav-iFluor 488. Data shown was gated on the CD11c+ population. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Mouse XCR1 Antibody (S-R601)
Biotin Mouse Anti-Mouse XCR1 Antibody (S-R601)
Price:
Regular price
$111.00 SGD
Regular price
Sale price
$111.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | XCR1 |
| Synonyms | Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; mXCR1; Ccxcr1; Xcr1 |
| Location | Cell membrane |
| Accession | Q9R0M1 |
| Clone Number | S-R601 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | collagenase-digested C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
XCR1 is a conserved, 37-kDa, seven-transmembrane, G-protein-coupled receptor selectively expressed on CD8α⁺ and CD103⁺ conventional dendritic cells; its only ligand, XCL1, is secreted by activated CD8⁺ T and NK cells to form a feed-forward loop that efficiently recruits antigen-bearing dendritic cells into draining lymph nodes, licenses cross-presentation, and amplifies cytotoxic immune responses against viruses and tumors, while genetic or antibody blockade of XCR1 impairs viral clearance, vaccine efficacy, and checkpoint-blockade-mediated anti-tumor immunity.
Picture
Picture
FC
