Flow cytometric analysis of Human GPR15 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with Alexa Fluor® 647 Mouse Anti-Human CD4 Antibody and either Biotin Mouse IgG2a, k Isotype Control (left panel) or SDT Biotin Mouse Anti-Human GPR15 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human GPR15 Antibody (S-3534)
Biotin Mouse Anti-Human GPR15 Antibody (S-3534)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | GPR15 |
| Synonyms | G-protein coupled receptor 15; Brother of Bonzo (BoB) |
| Location | Cell membrane |
| Accession | P49685 |
| Clone Number | S-3534 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Isotype Control | S0B5432 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
GPR15 is a class-A orphan G-protein-coupled receptor best known for guiding colon-specific homing of T cells, especially FOXP3+ Tregs and effector subsets, via its recently identified ligand C10orf99 (GPR15L), a 9 kDa chemokine-like peptide abundant in gut epithelia; beyond establishing intestinal immune homeitasis, GPR15 also serves as an HIV/SIV co-receptor, is up-regulated by TGF-β and the aryl hydrocarbon receptor, exhibits species-specific expression patterns, and has emerged as a potential therapeutic target for colitis and other mucosal inflammatory disorders.
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