Flow cytometric analysis of Human CD94 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with PE Mouse Anti-Human CD56 Antibody and either Biotin Mouse IgG1, κ Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD94 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD94 Antibody (HP-3D9)
Biotin Mouse Anti-Human CD94 Antibody (HP-3D9)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD94 |
| Synonyms | Natural killer cells antigen CD94; KP43; Killer cell lectin-like receptor subfamily D member 1; NK cell receptor; CD94; KLRD1 |
| Location | Cell membrane |
| Accession | Q13241 |
| Clone Number | HP-3D9 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B5431 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD94, encoded by KLRD1, is a type II transmembrane C-type lectin glycoprotein expressed predominantly on natural killer (NK) cells and subsets of CD8⁺ T cells, where it covalently assembles with NKG2A, C, E or F subunits to form disulfide-linked heterodimers that recognize the non-classical MHC-I molecule HLA-E (or Qa-1 in mice) and deliver either inhibitory or activating signals that finely tune cytotoxicity, cytokine secretion and survival of effector lymphocytes, thereby playing a central role in innate and adaptive immunity, tumor immune evasion, viral defense and pregnancy tolerance.
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