Flow cytometric analysis of CD8α expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Biotin Mouse IgG2a Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD8α Antibody (right panel) at 2 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD8α Antibody (OKT-8)
Biotin Mouse Anti-Human CD8α Antibody (OKT-8)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD8α |
| Synonyms | T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; MAL; CD8A |
| Location | Secreted, Cell membrane |
| Accession | P01732 |
| Clone Number | OKT-8 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Isotype Control | Biotin Mouse IgG2a Isotype Control (S-R454) |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood lymphocytes |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 2 μl per million cells in 100μl volume | Hu |
Background
CD8α is a crucial immune glycoprotein that plays a vital role in the immune response by serving as a co-receptor for the T-cell receptor (TCR) on cytotoxic T cells. It forms either a homodimer (α/α) or a heterodimer with CD8β (α/β) to interact with MHC class I molecules on antigen-presenting cells (APCs), facilitating the recognition of intracellular pathogens such as viruses. CD8α also recruits the LCK kinase, which initiates downstream signaling pathways essential for T-cell activation and proliferation. This molecule is critical for the function of cytotoxic T lymphocytes (CTLs) and is involved in various immune-related diseases, including immunodeficiencies and autoimmune conditions.
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