Flow cytometric analysis of Human CD73 expression on human peripheral blood cells. Human peripheral blood cells were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Biotin Mouse IgG1, k Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD73 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human CD73 Antibody (AD2)
Biotin Mouse Anti-Human CD73 Antibody (AD2)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD73 |
| Synonyms | 5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase; NT5; NTE; NT5E |
| Location | Cell membrane |
| Accession | P21589 |
| Clone Number | AD2 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B5431 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD73, also known as ecto-5'-nucleotidase (encoded by NT5E), is a glycosylphosphatidylinositol (GPI)-anchored cell-surface enzyme that catalyzes the final step of extracellular ATP degradation, converting AMP to immunosuppressive adenosine, thereby orchestrating tumor immune evasion, promoting angiogenesis and metastasis, and shaping an anti-inflammatory microenvironment by dampening CD8⁺ T-cell cytotoxicity and enhancing Treg or myeloid suppressive functions across multiple solid cancers.
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