Flow cytometric analysis of human CD54 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with either Biotin Mouse IgG2a Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD54 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD54 Antibody (S-3807)
Biotin Mouse Anti-Human CD54 Antibody (S-3807)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD54 |
| Synonyms | Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; ICAM1 |
| Location | Membrane |
| Accession | P05362 |
| Clone Number | S-3807 |
| Isotype | IgG2a |
| Isotype Control | S0B5432 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD54 is an 85–110 kDa transmembrane glycoprotein of the immunoglobulin superfamily that is expressed on endothelial, epithelial and immune cells; upon binding its ligands LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) it serves as the critical adhesion checkpoint that promotes firm leukocyte arrest on the vessel wall, enables subsequent diapedesis into inflamed tissue, and amplifies antigen-specific T-cell activation, thereby positioning it at the mechanistic center of inflammatory, autoimmune and anti-tumor immune responses.
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